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Zeocin 博来霉素

Description

Zeocin is an antibiotic in the bleomycin family. Its mode of action is to intercalate into the DNA and degrade it. The Zeocin resistance gene encodes a 13,665 dalton protein. This protein confers resistance to Zeocin by binding to the antibiotic and preventing it from binding DNA. Zeocin is effective on most aerobic cells and can be used for selection in mammalian cell lines, yeast, and bacteria. For Zeocin to be active, the salt concentration in the selection media must be less than 90 mM NaCl. The amount of Zeocin required for selection will vary with each cell type.

Concentration of Zeocin for Selection:

Organism

Zeocin Concentration

E. coli

25 µg/ml in low salt LB medium

Mammalian Cells

500-1000 µg/ml (varies with cell line)

Contents & Storage

Zeocin is provided in a solution with a concentration of 100 mg/ml. Zeocin should be stored at -20C in the dark. When stored correctly, the drug is guaranteed for 6 months from the date of shipment.

Ordering Information

Description

Quantity

Price()

Promotion()

Cat. no.

产地

Zeocin

1 g

2988

2540(现货)

R250-01

Invitrogen

5 g

13527

12174(三周)

R250-05

Invitrogen

 


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