Buffer
component for separation of low molecular
weight peptides
技术参数
产地：Sigma T0377
Synonym:N-[Tris(hydroxymethyl)methyl]glycine
Molecular Formula:(HOCH2)3CNHCH2CO2H
Molecular Weight:179.17
CAS Number:5704-04-1
Beilstein Registry Number:1937804
EG/EC Number:2271936
MDL number:MFCD00004277
PubChem Substance ID:24899884
Application
Tricine is one of the zwitterionic
amino acids known as Good buffers. It has a
useful buffering range of pH 7.4 - 8.8.
Tricine was first prepared by Good to serve
as a buffer for chloroplast reactions. The
name tricine comes from tris and glycine
from which it was derived.
Tricine has been used as an
electrophoresis buffer. With a lower pK
compared to glycine, it is more negatively
charged and it will migrate faster. Also,
the high ionic strength causes more ion
movement and less protein movement. Both
factors allow low molecular weight proteins
to be separated in lower percent acrylamide
gels. Lower gel concentrations permit easier
transfers to membranes. The use of tricine
in the separation of proteins in the range
of 1 to 100 kDa by electrophoresis has been
reported
For ATP assays using firefly luciferase,
tricine buffer at 25 mM was found to be the
best of ten common buffers tested.5 Tricine
was found to be an efficient scavenger of
hydroxyl radicals in a study of
radiationinduced membrane damage.
Precautions and Disclaimer
For Laboratory Use Only. Not for drug, household or other
uses.
Preparation Instructions
A buffer using a tricine stock solution may be prepared by
titrating with sodium hydroxide to the
desired pH.7 A saturated aqueous solution is
0.8 M at 0 °C
Storage/Stability
Tricine buffers may be autoclaved. The pH is identical before
and after autoclaving for 0.5 M solutions
(at pH 7.2, 8.0, and 9.2) and a 0.05 M
solution (at pH 7.9).
