Sigma T1375
assay
≥99% (titration)
Synonym:别名
2-[(2-Hydroxy-1,1-bis(hydroxymethyl)ethyl)amino]ethanesulfonic
acid
N-[Tris(hydroxymethyl)methyl]-2-aminoethanesulfonic acid
TES free acid
Molecular:FormulaC6H15NO6S 分子式
Molecular:Weight229.25 分子量
CAS Number:7365-44-8 Beilstein Registry Number 1957061
EG/EC Number:2309063
MDL number:MFCD00007532
PubChem Substance ID:24899960
STORAGE / STABILITY AS SUPPLIED:
TES free acid is stable at room temperature for at least
three years.
SOLUBILITY / STABILITY OF SOLUTIONS:
TES free acid is very soluble in water; T1375, routinely
tested for solution appearance at 33% (w/v), gives a clear
colorless solution. T6541, SigmaUltra, gives a clear
colorless solution at 1 M, with a pH range of 3.5-5.0.3
Solutions should be stable at 2-8EC for at least six months.
Sterilization should be done by filtration through 0.2 m
filters, since generally autoclaving is not recommended for
any sulfonic acid buffers. However, autoclavability was
checked on 0.5M solutions at pH 6.7, 7.45 and 8.5, and at
0.05M at pH 7.4. The pH was the same between autoclaved and
non-autoclaved samples and solutions remained colorless.
GENERAL REMARKS:
TES is a structural analog to Trizma buffer, one of the
ethanesulfonic acid series of biological buffers developed
by Good et al., to meet these criteria: midrange pKa,
maximum water solubility and minimum solubility in all other
solvents,minimal salt effects, minimal change in pKa with
temperature, chemically and enzymatically stable, minimal
absorption in visible or UV spectral range, and easily
synthesized. Although the structure above is drawn as a
neutral molecule, it probably exists in solution as a
zwitterion.
Because the pKa is 7.4 (physiological pH) this buffer has
considerable potential for a variety of biological
applications.In many culture media that require metal
cations, many buffers cannot be used due to chelation or
precipitation (citrateor phosphate, for example.) TES was
found to be particularly beneficial in a study of succinate
oxidation.
A buffer using TES free acid can be prepared by titrating the
free acid (T1375 or SigmaUltra T6541) with sodium hydroxide
to the desired pH, using about a half-equivalent of NaOH.
Alternatively, solutions of equimolar TES free acid and TES
sodium (T0772) can be mixed to attain a buffer of the
desired pH. Titrating a solution of TES sodium with HCl is
not recommended since the resulting solution will contain a
half-equivalent of NaCl.
Sigma offers a number of TES buffer products: T1375 (reagent
grade), T6541 (SigmaUltra tested for trace metal
impurities), T6022 (cell culture-tested), and T4152
(molecular biology-tested). T1030, a hemisodium TES,
dissolves in water to produce a buffer with pH approximately
7.4. Please check current catalog listings.
