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Custom Immunology Services
In Vitro Production

In vitro production of monoclonal antibodies has increasingly become a more viable option for researchers. With more than 15 years of tissue culture experience, Equitech-Bio, Inc.  Immunology Services has successfully employed numerous in vitro production methods. Our goal is simple: Match the characteristics of your cell line with the optimum production method, at an affordable cost. Equitech-Bio has the experience and knowledge to achieve this goal, for both small and large-scale production runs, with the assurance of quality standards required by the research community. Many investigators choose to follow up with Protein A or Protein G purification to isolate the 免疫球蛋白 and concentrate the antibody. Any historically successful media formulation will provide for optimal cell growth. If the formulation is not known, Equitech-Bio鈥檚 staff must determine optimal cell growth conditions. This may lengthen production time.

We offer scale-up in vitro antibody production in flask or bioreactors as the final phase of a custom monoclonal project.  As an alternative, you may provide an established hybridoma cell line for a production run. All materials sent in for production should be mycoplasma tested.

 

Equitech-Bio also offers off-site storage as well as the generation of Master and Working Cell Banks for your cell line. Each submitted cell line undergoes a series of growth, viability, and mAb production assays that is reported in your custom Cell Profile Sheet.

We offer the following scale-up technologies:

  • Flask 鈥 Scale up of your antibody through flask production eliminates the possibility of interfering murine proteins as can be found with in vivo methods. The result is a cleaner antibody preparation.
  • Hollow fiber 鈥 Equitech-Bio, an industry leader, offers Hollow Fiber Technology for production of milligram to grams quantities of monoclonal antibodies. Our vast experience enables us to optimize your monoclonal cell line for maximum yields in cell growth and secreted protein production.
    Although hollow fiber is an established technology, significant advances in fiber materials have greatly improved productivity allowing for denser cell growth. Benefits of hollow fiber production include the ability to produce high concentration quantities of mAbs, elimination of murine contaminants, as well as the ability to adapt cells to serum free-media.
  • Small Bioreactors 鈥 These small bioreactors are a membrane based cultivation system intended for scale up of your antibody at a higher concentration than can be achieved through flask production.
  • Large Scale 鈥 Large scale bioreactors are designed for large scale cell culture production. They are a good alternative for cells that like static environments and provide an increased surface area which ranges from 632 cm2 to 25,284 cm2.

A.  Flask productionxe "Flask production" (cell produced culture fluid)xe "cell produced culture fluid"

100 ml            $2500

250 ml            $4500

500 ml            $6000

1.0 liter           $10,000

 

B.  Hollow fiber bioreactor productionxe "Hollow fiber bioreactor production" (cell produced culture fluid)xe "cell produced culture fluid"

1 to 5 liters     $10,000/Liter

6 to 10 liters   $9000/Liter

11+ liters        $8000/Liter

Note:  Flask and bioreactor production quotes are based on RPMI 1640 @ 5% fetal bovine serum as the standard culture medium.  If your cell line requires specialized medium formulation, please inquire.

 

     C.  Purification of antibody

 We purify the antibody by either Protein A or by our proprietary chemical method.  The solution is concentrated to 10 or 40 mg of protein per ml in 10 mM Phosphate, 0.150M NaCl, pH 7.2 buffer unless otherwise stated.  Price includes sterile filtration and a certificate of analysis.

 0.1 mg to 100 mg     Please Inquire

250 mg                       Please Inquire

500 mg                       Please Inquire

  1 g                             Please Inquire

  2 g                             Please Inquire

  5 g                             Please Inquire

10 g                            Please Inquire

 


 

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