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cellsignal公司产品
Cleaved Caspase-3 (Asp175) Antibody #9661 
 

No. Size Price    
9661L 300 ul ( 30 Western mini-blots ) $629 In Stock 两周到货
9661S 100 ul ( 10 Western mini-blots ) $262 In Stock 现货
custom custom/drug discovery tbd   email request
Applications Reactivity Sensitivity MW (kDa) Source
W IHC-P IHC-F IF-IC IC F H M R B (Pg) Endogenous 17, 19 Rabbit

Applications Key:  W=Western Blotting  IHC-P=Immunohistochemistry (Paraffin)  IHC-F=Immunohistochemistry (Frozen)  IF-IC=Immunofluorescence (Immunocytochemistry)  IC=Immunocytochemistry  F=Flow Cytometry
Reactivity Key:  H=Human  M=Mouse  R=Rat  Pg=Pig  B=Bovine
Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

Cleaved Caspase-3 (Asp175) Antibody detects endogenous levels of the large fragment (17/19 kDa) of activated caspase-3 resulting from cleavage adjacent to Asp175. This antibody does not recognize full length caspase-3 or other cleaved caspases.

Source / Purification

Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to amino-terminal residues adjacent to (Asp175) in human caspase-3. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa, NIH/3T3 and C6 cells untreated, staurosporine-treated (1 µM in vivo) or cytochrome c-treated (0.25 mg/ml in vitro), using Caspase-3 Antibody #9662 (upper) or Cleaved Caspase-3 (Asp175) Antibody (lower).

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human tonsil, showing cytoplasmic and perinuclear localization in apoptotic cells (low and high magnification), using Cleaved Caspase-3 (Asp175) Antibody.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded Jurkat cells, untreated (left) or etoposide treated (right), using Cleaved Caspase-3 (Asp175) Antibody.


 

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded mouse embryo, using Cleaved Caspase-3 (Asp175) Antibody preincubated with control peptide (left) or Cleaved Caspase-3 (Asp175) Blocking Peptide #1050 (right).

IHC-F (frozen)

IHC-F (frozen)

Immunohistochemical analysis of frozen H1650 xenograft section, using Cleaved Caspase-3 (Asp175) Antibody.

IHC-FL (floating)

IHC-FL (floating)

Confocal micrograph of newborn rat brain tissue, showing control and transient cerebral ischemia, using Cleaved Caspase-3 (Asp175) Antibody (green) and propidium iodide (red). (Provided by Dr. Bingren Hu, University of Miami School of Medicine, Florida.)


 

IHC-FL (floating)

IHC-FL (floating)

Confocal micrograph of newborn rat brain cortex (Cx) and striatum (Str), using Cleaved Caspase-3 (Asp175) Antibody (green) and propidium iodide (red). (Provided by Dr. Bingren Hu, University of Miami School of Medicine, Florida.)

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of Jurkat cells, untreated (blue) or etoposide-treated (green), using Cleaved Caspase-3 (Asp175) Antibody compared to a nonspecific negative control antibody (red).

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of Pam212 cells, untreated or sulindac sulfone-treated (to induce apoptosis), using Cleaved Caspase-3 (Asp175) Antibody. Results were similar to those obtained by analyzing DNA content. (Provided by Dan Rosson, Ph.D., Lankenau Institute of Medical Research, Wynnewood, PA.)


 

IF-IC

IF-IC

Confocal immunofluorescent images of HT-29 cells, untreated (left) or Staurosporine #9953 treated (right), labeled with Cleaved Caspase-3 (Asp175) Antibody (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).

Background

Caspase-3 (CPP-32, Apoptain, Yama, SCA-1) is a critical executioner of apoptosis, as it is either partially or totally responsible for the proteolytic cleavage of many key proteins such as the nuclear enzyme poly (ADP-ribose) polymerase (PARP) (1). Activation of caspase-3 requires proteolytic processing of its inactive zymogen into activated p17 and p12 fragments. Cleavage of caspase-3 requires aspartic acid at the P1 position (2).

 

  1. Fernandes-Alnemri, T. et al. (1994) J. Biol. Chem. 269, 30761-30764.
  2. Nicholson, D. W. et al. (1995) Nature 376, 37-43.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

  • 1050 Cleaved Caspase-3 (Asp175) Blocking Peptide
  • 9662 Caspase-3 Antibody
  • 9852 Chaps Cell Extract Buffer (10X)
  • 7074 Anti-rabbit IgG, HRP-linked Antibody
  • 7720 Prestained Protein Marker, Broad Range (Premixed Format)
  • 7727 Biotinylated Protein Ladder Detection Pack
  • 7003 20X LumiGLO® Reagent and 20X Peroxide
  • 8104 SignalSlide™ Cleaved Caspase-3 (Asp175) IHC Controls

This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.


 


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