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chaps双向电泳实验常用试剂 chaps, 分子式为C32H58N2O7S, 分子量为614.88,白色粉末,常温保存。CHAPS是两性离子去垢剂,分子结构中具有一个胆酸和硫代甜菜碱。在紫外区的低吸光性成为用紫外方法检测膜蛋白的首选去垢剂,CMC值均为8 mM。常用于于双向电泳等实验。 原文简介:CHAPS is a nondenaturing zwitterionic detergent for membrane biochemistry.Useful for solubilizing membrane proteins and breaking protein-protein interactions. CHAPS’ small micellar molecular weight (6,150) and high critical micelle concentration (6-10 mM) allow it to be removed from samples by dialysis. It is also suitable for protein solubilization for isoelectric focusing and two-dimensional electrophoresis. CHAPS is commonly used for non-denaturing (without urea) IEF and has been shown to give excellent resolution of some subcellular preparations and plant proteins. Concentrations between 2-4% (w/v) are typically used in an IEF gel. 技术参数:
包装与优惠价:580元/5g 250元/1g
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