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                      Annexin V试剂盒                               更多调亡试剂

    ----细胞膜改变的检测

    Merck公司提供的Annexin V试剂盒特有的RAPIDTM Media Binding Reagent可直接将Annexin V-Fitc快速标记于培养液中的细胞上,避免额外的离心、清洗等可能造成细胞膜机械性破坏的操作。此外,一旦细胞离开培养环境,细胞调亡即成为渐进性的动态过程,会持续整个试验操作,过于频繁的操作步骤往往会错过检测早期调亡的最佳时间,使用试剂盒中持有的RAPIDTM Media Binding Reagent能简化操作、缩短试验操作的时间,更有利于检测早期调亡的细胞。试验结果可用流式细胞仪或荧光显微镜检测。可用于多种种属来源细胞的检测。

Format: Flow cytometry or fluorescence microscopy

Form: 100 Tests

Detection Method: Fluorometric

Species Reactivity: a broad range of species

Assay Time: 15 - 30 min

Sample Type: Intact cells

Positive Control: Apoptotic Jurkat cells (dexamethasone or anti-Fas treated)

Kit Contents: Annexin V-FITC, 5X Binding Buffer, Propidium Iodide, RAPID™ Media Binding Reagent, and a user protocol.

Comments: Optimized for flow cytometry and fluorescence microscopy applications. A RAPID™ protocol has been developed for Annexin V-FITC binding directly in tissue culture media. This obviates the need for tedious centrifugation and wash steps, that increase the occurrence of mechanical membrane disruption. In addition, since apoptosis is a dynamic process that is ongoing once cells are removed from culture conditions and continues throughout experimental processing, the RAPID™ protocol is recommended for the detection of cells in early apoptosis.

Ref.: Frey, T. 1997. Cytometry 28, 253. Darzynkiewicz. Z., et al. 1997. Cytometry 27, 1. Boersma, A.W.M., et al. 1996. Cytometry 24, 123. Wyllie, A. H. 1993. Br. J. Cancer 67, 205. Darzynkiewicz, Z., et al. 1992. Cytometry 13, 795. Fadok, V.A., et al. 1992. J. Immunology 148, 2207. Kerr, J.F.R., et al. 1972. Cancer 26, 239.

R: 36; S: 22-24

Sold under license of U.S. Patent 5,834,196.

FITC Log Plot. Viable cells do not bind Annexin V-FITC or Propidium Idide (PI) as reflected in the lower left-hand quadrant of the dot plot. Early apoptotic cells with exposed PS but intact cell membranes bind Annexin V-FITC but exclude PI. Fluorescence from this population is reported in thelower right-hand quadrant. Necrotic or apoptotic cells in terminal stages will be both Annexin V-FITC and PI positive and will be reported in the upper right-hand quadrant. A small percentage of normal cell death should be expected in routine cultures of untreated cells.
更多产品信息:http://www.merckbiosciences.com/product/PF032

产品订购:

名称 目录号 应用 样本类型 阳性对照 反应次数
Annexin V-FITC Apoptosis Detection Kit PF032 IF,FC 完整细胞 100 Tests

 

相关产品:

Annexin V-Biotin Apoptosis Detection Kit  用于检测早期调亡的细胞膜改变,可连接GFP或其它荧光素    

Annexin V-Biotin Apoptosis Detection Kit II 用于检测早期调亡的细胞膜改变,可连接GFP或其它荧光素

Annexin V-FITC Apoptosis Detection Kit II  用于检测早期调亡的细胞膜改变

Annexin V-PE Apoptosis Detection Kit  用于检测早期调亡的细胞膜改变

 

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