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BICINE 生物缓冲液
Sigma
B8660 assay≥99%
(titration) Bicine is a zwitterionic buffer used in biochemistry and molecular biology research. Originally prepared in the 1920's, it later became one of the Good buffers that were studied in the 1960's to provide buffers in the pH range of 6.15 - 8.35 for wide applicability to biochemical studies.
The use of bicine in a thin layer ion exchange
chromatography method for protein resolution has been
published.3 Bicine has been utilized in peptide and protein
crystallization.4,5,6 A kinetic study of a quaternary
transition-state analogue complex of creatine kinase used
bicine in the reaction buffer.7 A multiphasic buffer system
for SDS-PAGE of proteins and peptides that incorporates
bicine has been described.8
Preparation Instructions |



