| Recombinant Enterokinase
(rEK) is a highly purified preparation of
the catalytic subunit of bovine enterokinase,
a serine protease which recognizes the
identical cleavage site as the native enzyme
(AspAspAspAspLys↓) and has similar enzymatic
activity. This enzyme is produced in E.
coli and purified to yield the highest
activity available, and is qualified for
specific cleavage of appropriate fusion
proteins. Supplied as a solution in 50%
glycerol. EC 3.4.21.9, M.W. 26,300.
References:
Collins-Racie, L.A. et al. 1995.
Bio/Technology 13, 982.
Unit Activity:
One unit is defined as the amount of enzyme
that will cleave 50 µg of fusion protein in
16 hours at 23°C, in a buffer containing 20
mM Tris-HCl pH 7.4, 50 mM NaCl, 2 mM CaCl2.
Components:
|
• |
50 U |
Recombinant
Enterokinase
|
|
• |
2 ml |
1X rEK
Dilution/Storage Buffer
|
|
• |
1 ml |
10X rEK Cleavage
Buffer
|
|
• |
10 µg |
Cleavage Control
Protein |
|
