Platinum® Taq DNA Polymerase High Fidelity

Description:

Platinum® Taq DNA Polymerase High Fidelity is ideal for amplification of DNA fragments where high yields and robust amplification are required. High fidelity is provided by a mixture of Platinum® Taq DNA Polymerase and the     View More

Platinum® Taq DNA Polymerase High Fidelity is ideal for amplification of DNA fragments where high yields and robust amplification are required. High fidelity is provided by a mixture of Platinum® Taq DNA Polymerase and the proofreading (3´a5´ exonuclease activity) enzyme Pyrococcus species GB-D polymerase (Table 1). PCR specificity is improved with the incorporation of Platinum® automatic "hot-start" technology. With Platinum® Taq High Fidelity you'll get:

• Greater than six times higher fidelity than Taq DNA poly-merase

• Amplification of fragments up to 15 kb (Figure 1)

• Room temperature reaction assembly

Applications: Amplification of DNA from complex genomic, viral, and plasmid templates; and RT-PCR (1). Platinum® Taq High Fidelity is especially effective for high-fidelity applications such as cloning or mutagenesis.

Unit Definition: One unit of Platinum® Taq DNA Polymerase High Fidelity is the amount of enzyme required to incorporate 10 nmoles of deoxyribonucleotide into acid-precipitable material in 30 min. at 74°C.

Contents and Storage:
Platinum® Taq DNA Polymerase High Fidelity is supplied with a vial of 10X High Fidelity Buffer [600 mM Tris-SO₄ (pH 8.9), 180 mM (NH₄)₂ SO₄ ], and a vial of 50 mM MgSO₄ . Platinum® PCR SuperMix High Fidelity is supplied in a 1.1X concentration and contains Platinum® Taq DNA Polymerase High Fidelity, dNTPs, salts, and MgSO₄ . Store at -20°C. Guaranteed stable for 6 months when properly stored.

Ordering Information

名称	规格	报价	折后价	货号	产地
Platinum® Taq DNA Polymerase High Fidelity	500 rxns	9375	8568现货	11304-029	Invitrogen

 

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