CIAP，催化磷酸单脂的水解，消化DNA、RNA、核糖核苷核脱氧核糖核苷，释放磷酸基因。此外也可以除去蛋白上的磷酸基因。
Calf Intestine Alkaline Phosphatase
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#EF0341
Supplied with:
10X Reaction Buffer |
200u (1u/µl)
1ml |
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#EF0342
#EF0343
Both supplied with:
10X Reaction Buffer |
5x200u (1u/µl)
HC, 1000u (10u/µl)
5x1ml |
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Related Documents (in pdf,
~40-60KB):
Certificate of Analysis:
#EF0341,
#EF0342,
#EF0343
MSDS |
Description
Calf Intestine Alkaline Phosphatase (CIAP) catalyzes the
release of 5'- and 3'-phosphate groups from
DNA, RNA, deoxyribonucleoside and
ribonucleoside mono-, di- and triphosphates.
Also this enzyme can remove phosphate groups
from proteins.
Source
Calf intestine.
Feature
Active in
Fermentas buffers for restriction
enzymes, PCR and RT.
Applications
Quality Control
Tested for the
absence of endo-, exodeoxyribonucleases,
ribonucleases and for dephosphorylation of
DNA 5'termini.
Concentration
1u/µl
10u/µl, HC
Definition of Activity Unit
One unit of the enzyme hydrolyzes 1 micromole of
4-nitrophenylphosphate in 1min at 37°C.
Enzyme activity is assayed in the following
mixture: 1M diethanolamine-HCl (pH 9.8),
0.5mM MgCl2 and 10mM 4-nitrophenylphosphate.
Storage Buffer
The enzyme is supplied in: 20mM Tris-HCl (pH 8.0), 1mM MgCl2,
50mM KCl, 0.1mM ZnCl2 and 50% (v/v)
glycerol.
10X Reaction
Buffer,0.1M Tris-HCl (pH 7.5 at 37°C), 0.1M
MgCl2.
Inhibition and Inactivation
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Inhibitors: metal chelators, inorganic
phosphate and phosphate analogs.
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Inactivated by heating at 85°C for 15min
or at 75°C for 10min in the presence of
5mM EDTA (pH 8.0), or by
phenol/chloroform extraction.
Activity in Buffers for Restriction Enzymes,
PCR, RT
References
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Ausubel, F.M., et al., Current Protocols
in Molecular Biology, vol. 1, John Wiley
& Sons, Inc., Brooklyn, New York,
3.10.1-3.10.2, 1994-2004.
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Ahmad, Z., Huang, K.P.,
Dephosphorylation of rabbit skeletal
muscle glycogen synthase (phosphorylated
by cyclic AMP-independent synthase
kinase 1) by phosphatases, J. Biol.
Chem., 256, 757-760, 1981.
现货供应，150元/200U 浓度为1U/ul
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