HEPES SODIUM SALT，也称为HEPES-Na中文名称4-羟乙基哌嗪乙磺酸钠HEPESNa，分子式:C8H17N2O4SNa，分子量为:260.3，CAS:
75277-39-3，白色晶体粉末。
HEPES SODIUM SALT作用：（英文原文）
This product is designated as Biotechnology Performance Certified (BPC)
reagent grade and meets the specifications of the USP, EP, and JP for
reagent chemicals. In addition, this product can be used for molecular
biology, cell culture, and electrophoresis applications.
HEPES does not bind magnesium,
calcium,manganese(II), or copper(II) ions.4
HEPES has been described as one of the best
allpurpose buffers available for biological research.3 At most
biological pHs, the molecule is zwitterionic, and is most effective as a
buffer at pH 6.8 to 8.2 (pK±1, as a general rule). HEPES has been used in a
wide variety of applications, including tissue culture.
Buffer strength for cell culture
applications is usually in the range of 10 to 25 mM. Care must be taken to
maintain appropriate osmolality in media, and toxicity with respect to a
given cell line must be evaluated.Isotonicity data have been tabulated.5
HEPES is reportedly superior to NaHCO3 in controlling pH in tissue and organ
culture.6
Unfortunately, HEPES is not recommended for
certain protein applications. It interferes with the Folin-Ciocalteu protein
assay. The Biuret protein assay isunaffected.7
HEPES was the buffer of choice in a protein
deposition technique in electron microscopy because it did not affect metal
substrates.9 HEPES was evaluated and shown to be quite suitable
for use with Ampholines
in generating pH gradients less than 1 pH unit wide for isoelectric focusing
applications.10
产品参数：
HEPES SODIUM SALT
(N-[2 HYDROXYETHYL] PIPERAZINE -N'-[2-ETHANESULFONIC ACID])
High Purity Grade
C8H17N2O4SNa MW: 260.3 [75277-39-3]
Purity (Dry Basis) (%)
.................................
≥99.0
Loss on Drying (%)
.......................................≤3.0
pKa @XX°C
.........................................7.45
- 7.65
Heavy Metals (as Pb) (%)
..............................<
0.0001
Solubility (5.0%, Water)
..................................PASS
DNase
............................................None Detected
RNase
............................................None Detected
Protease
.........................................None Detected
Storage at RT Amresco 0485
订购信息：
|
货号 |
品名 |
规格 |
市场价（元） |
促销价（元） |
产地 |
|
0485 |
HEPES SODIUM SALT |
25g |
250 |
无促销 |
Amresco |
|
0485 |
HEPES SODIUM SALT |
100g |
764 |
650 |
Amresco |
|
0485 |
HEPES SODIUM SALT |
500g |
3175 |
2700 |
Amresco |
|
0485 |
HEPES SODIUM SALT |
1kg |
5715 |
1850 |
Amresco |
|
0485 |
HEPES SODIUM SALT |
25kg |
12144375 |
寻价，现货 |
Amresco |
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